In-vitro Anti-Ulcer Activity of Ethanolic Extract of Tecoma Stans Flower

Abstract

Aims: The present in-vitro investigation assessed the anti-ulcer potential of Tecoma stans flower extract through acid neutralization capacity assay and Proton pump inhibitor (H+/K+-ATPase) assay and preliminary phytochemical analysis. Study design: Experimental laboratory based invitro study. Methods: Flower extract of Tecoma stans was screened for phytochemical constituents. Anti-ulcer property was assessed by using acid neutralization capacity assay andProton pump inhibitor (H+/K+-ATPase) inhibition method. The percentage inhibition, the opticaldensity at 660nm and IC50 values of H+/K+-ATPase inhibition method were determined. Results: The preliminary phytochemical constituents of the extract was identified as alkaloids, flavonoids, phenolic compounds, saponins and tannins indicating that the bioactive constituents are capable of neutralize stomach acid. The acid neutralizing capacity assay revealed that the dose -dependent increase with the extract closely comparable to the standard dose and the H⁺/K⁺-ATPase inhibition assay demonstrated concentration-dependent enzyme inhibition with an IC50 value confirming moderate proton pump inhibitory activity. Conclusion: These findings suggest that Tecoma stans contains potent bioactive phytochemicals capable of modulating ulcer pathways through acid neutralization capacity assay and proton pump inhibitor (H+/K+-ATPase) inhibition method.