Evaluation of Antioxidant Activity Gomphrena Celosioides Leaf Extract by Using Scavenging Methods

Abstract

Aim: In the current in vitro study, the antioxidant potential of Gomphrena celosioides leaf extract was evaluated by using the hydrogen peroxide scavenging method and the DPPH radical scavenging Method. Study design: Experimental laboratory-based in vitro study. Method: Extraction and Investigation of Gomphrena celosioides leaf was done for different phytochemical constituents, and the Antioxidant potency was examined by using the hydrogen peroxide scavenging method (absorbance measured at 230 nm) and the DPPH radical scavenging method (absorbance measured at 517 nm). Percentage inhibition, minimum inhibition, maximum inhibition, and IC50 values were calculated. Results: Phytochemical investigation shows the presence of resins, carboxylic acid, steroids, saponin, and alkaloids; thus, all phytochemical constituents have a certain degree of antioxidant activity by donating the hydrogen atom to the free radicals and neutralizing them. The results confirm that the ethanolic extract of Gomphrena celosioides leaf has a dose-dependent antioxidant property. And the IC50 value suggested the extract had moderate to strong antioxidant properties. Conclusion: Here, the ethanolic extract of Gomphrena celosioides leaf exhibits moderate to strong antioxidant activity that has been consistently attributed to the presence of phytochemical constituents like resins, carboxylic acid, steroids, saponin, and alkaloids.