In-Vitro Antioxidant Evaluation of Crassula ovata Leaf Extract Using DPPH Radical Scavenging Assay and Phytochemical Profiling

Abstract

Aims:
The present in vitro investigation assessed the antioxidant potential of Crassula ovata leaf extract through DPPH radical scavenging and preliminary phytochemical analysis.

Study Design:
Experimental laboratory-based in vitro study.

Methods:
Leaf extract of C. ovata was screened for major phytochemical constituents. Antioxidant capacity was examined using the DPPH radical scavenging method by measuring absorbance changes at 517 nm across different extract concentrations. Percentage inhibition and IC₅₀ values were calculated to determine potency.

Results:
Phytochemical screening revealed the presence of alkaloids, steroids, saponins, and resins, indicating the presence of bioactive constituents capable of donating hydrogen atoms or electrons to neutralize free radicals. C. ovata demonstrated concentration-dependent scavenging of DPPH radicals, with percentage inhibition increasing progressively with dose. The calculated IC₅₀ value suggests moderate to strong antioxidant potential for the plant extract.

Conclusion:
The antioxidant activity exhibited by Crassula ovata may be attributed to its phytochemical constituents. These findings support the plant’s potential application as a natural source of antioxidant compounds.